10000CS465122101

PART ட்‌
Answer any two full questions,each carries 9 marks.

14 a) Using Needleman and Wunsch dynamic programming method, construct the (9)

15 a)

16

17
18

19

a)

b)

a)

a)

b)

partial alignment score table for the following two sequences, using the

following scoring parameters: match score: +1, mismatch score: -1,

gap penalty: -2.

Seql- ACGTCATCA

Seq2- TAGTGTCA

Write down the optimal global alignment between these sequences along with

optimal score.

Use UPGMA method to reconstruct a phylogenetic tree using the following (9)
distance matrix.

Species

Explain various steps involved in the construction of PAM matrix with an (5)

example.
Explain the applications of HMM in bioinformatics (4)
PART 0
Answer any two full questions, each carries 12 marks.
Differentiate prokaryotic and eukaryotic gene structure with a neat diagram. (12)

Explain the Chou-Fasman and GOR method for protein secondary structure (12)
prediction

Predict the secondary structure of given RNA sequence using Nussinov (6)
Algorithm

GGGAAAUCC.

Define transposition. Compare Retrotransposons and DNA transposons. (6)

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