D 00000CH407121901

Reg No.: Name:
APJ ABDUL KALAM TECHNOLOGICAL UNIVERSITY

Pages: 1

Seventh Semester B.Tech Degree Examination (Regular and Supplementary), December 2020

Course Code: CH407
Course Name: BIOCHEMICAL ENGINEERING

Max. Marks: 100 Duration: 3 Hours
PARTA
Answer any two full questions, each carries 15 marks. Marks
1 a) Describe the structure of a eukaryotic cell with a diagram. (10)
b) Compare competitive and uncompetitive inhibition. (5)
2 a) Derive the expression for Michaelis-Menten kinetics for a single-substrate (10)
enzyme catalysed reaction.
b) Write short note on cell fractionation. (5)
3 ஐ Explain polysaccharides with examples. (8)
b) Describe feedback inhibition of enzymes. (7)
PART B
Answer any two full questions, each carries 20 marks.
4 a) Describe enzyme immobilization. Explain the physical methods used for enzyme (10)
immobilization.
b) Describe the Embden-Meyerhof-Parnas (EMP) pathway. (10)
5 a) Differentiate between hydrolytic and proteolytic enzymes. List out the industrial (10)
applications of the same.
b) Describe the pentose-phosphate pathway. (10)
6 a) Describe transport of biomolecules in and out of a cell. (10)
b) List out and explain five medical applications of enzymes. (10)
PART ட்‌
Answer any two full questions, each carries 15 marks.
7 2) Write short note about a fed -batch reactor? (5)
b) Describe the different stages of a typical batch growth curve. (10)
8 a) With a neat schematic diagram explain the steps involved in the transport of (10)
oxygen from a gas bubble to inside of a cell. Describe the resistances involved.
b) What is a hemocytometer? How is it used? (5)
9 a) Describe the mass transfer across a gas liquid free surface. (6)
b) Describe Gaden’s classification of fermentation schemes. (9)

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