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2 C 20219

Example for type II restriction endonuclease is :

(a) Hind II. (9) Eco Bl.
(c) Eco Pl. (d) Hin fil.
In Sanger’s DNA sequencing method the chain termination is achieved by using ——————- sugar.

A transgenic animal in which a gene has been replaced or disrupted with an artificial DNA is
called ————__,

(12 x %=6 marks)
Section B

Answer all questions.

Each question carries 2 marks.
Pedigree chart.
Allelomorph.
Pleiotropism.
Headful packaging mechanism.
Spindle fibres.
Cdk inhibitors.
Liquid shear technique.
Primer.

Exo-nucleases.

Cosmids.
(10 x 2 = 20 marks)
Section C
Answer any six questions.
Each question carries 5 marks.
Extra-chromosomal inheritance.

Mechanism of transformation in Gram positive bacteria.
Celf cycle.

Use of artificial chromosomes in cloning.

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